Assignments
A. Notebook 25%
B. Presentation I (Technique/instrumentation) 20%
C. Presentation II 20%
D. Exam 25%
E. Quizzes and Assignments 5%
F. Wall Micrograph 5%
Prior to Admittance
A. Preliminary Project Description Due
Each student must submit a short project proposal prior to the onset of lab work. This must be submitted electronically.
B. Project Information Sheets Due
Each student must be identified with an account number to track usage within the CMRF. Some students may already have such an account.
September 4
Project Planning Paper Due
All aspects of your project need to be reviewed and planned. You need to determine where inconsistences can happen. Variations in fixation time, section thickness all can affect the results. With your CMRF staff instructor, Preplan your experiments. You will write a short plan detailing the following procedures:
Specimen collection
Fixation and processing
Sectioning
Staining
Image Collection
Analysis
September 16
A. Stereology homework due.
B. Topics for Presentation I due and approved by instructor
Each student will present a topic outside the scope of this course. The topic MUST be approved by the instructor by this date.
October 16 **********MIDTERM**************
A. All equipment training should be completed
1. Additional training can be provided at student expense.
2. Microscopes should be used during off-hours going forward.
B. Poster information due
If submitting a poster in leu of the notebook, you must have submitted the abstract and faculty sponsor letter.
October 28
Presentation I: Technique/instrumentation
Each student will give a 10-15 minute talk about current instrumentation and techniques that beyond the scope of this course
November 11
EXAM
November 18
A. Informal Power Point Presentation of Project Results with Micrographs
Each student will prepare a Power Point presentation of their research containing the micrographs generated thus far.
December 9
A. Presentation II: Research Presentations-Power Point
Each student will present a 10-minute PowerPoint talk describing students' project results and presenting high quality micrographs.
B. Notebooks due in 76 EMRB by 5:00 PM
Notebook Criteria and Grading
Each student will submit a notebook consisting of a short "paper" describing work done on their project.
The paper should contain an introduction stating project goals, a materials and methods section, and a results/discussion section
containing analysis and conclusions with supporting micrographs. Notebooks must be presented electronically.
A minimum of ten different high quality micrographs will be presented in the notebook at the end of the course.
1. All project micrographs are to be of high contrast, well-focused, free of knife marks and contamination.
Fluorescent images should not be over-saturated. The images should support your conclusions.
2. Each print must be accompanied by a sample description (legend).
3. Each print must have a marker bar to indicate scale (i.e., 1 mm or 0.01 mm etc.).
C. Wall Micrograph Due
Each student will choose one micrograph for artistic content and mount it for display in the CMRF.
• The image must be inserted into a template provided by the instructor.
• The micrograph should include a scale bar, a short legend and the names of the department, student and advisor.
• The image must be printed at high resolution and mounted on a black mounting board measuring 8 inches by 10 inches.
• The final image must be saved in .tif format and given to the instructor in electronic form.
