Notice: The IVIS system is now administered by the Small Animal Imaging Core (SAIC) and is located in room 120 PBDB

 

For information and access please contact the SAIC at saic@uiowa.edu

OPERATING INSTRUCTIONS

Instrument Description

The Xenogen IVIS-200 System is a high-sensitivity, low noise, in vivo imaging technology platform that allows non-invasive visualization and tracking of cellular and genetic activity within a living organism, in real time. It’s capable of imaging bioluminescence and fluorescence. IVIS-200 provides Living Image® software for image display and analysis.

Instrument Specifications

• CCD Cameras
  • Sensor
    • Back Illuminated
  • CCD Format
    • 2048 x 2048 pixels
  • CCD Size
    • 26 x 26 mm
  • Effective CCD Format
    • 1920 x 1920 pixels
  • Pixel Size
    • 13.5 x 13.5 um
  • Quantum Efficiency
    • ~85% 400-700 nm
      • >50% 350-900 nm
    • Readout Noise
      • <5 electrons RMS
    • Dark Current
      • <100 electrons/s/cm2 (-105°c)
    • Minimum Detectable Luminance
      • <70 photons/s/sr/cm2
    • CCD Temperature
      • Nominal ~105°C
  • Optics
    • Lens f/stop
      • f/1~f/8
    • Field of views (FOV)
      • 3.9, 6.5, 13, 19.5 and 26 cm
    • Resolution
      • >60 um (FOV=3.9, f/1)
    • Scanning Laser
      • <1 mW at 532 nm
  • Fluorescence Imaging Components
    • Excitation Filters
      • 6 positions, 25 mm diameter
    • Emission Filters
      • 22 positions, 60 mm diameter
    • Background (Autofluorescence and Leakage)
      • 1 part in 107 typical
    • Lamp
      • 150 W Quartz Halogen 21 V 3250° Kelvin

  There are fluorescence filter sets available on the system are as follows:

  Filter Set	Label	Excitation	Emission Passband	Dyes & Passband
  1	GFP	445-490	515-575	GFP, EGFP, FITC
  2	DsRed	500-550	575-650	DsRed2-1, PKH26, CellTrackerOrange
  3	Cy5.5	615-665	695-770	Cy5.5, Alexa Fluor® 660, Alexa Fluor® 680
  4	ICG	710-760	810-875	Indocyanine green (ICG)