Xenogen IVIS-200

Eckstein Medical Research Building - Room 87

 

OPERATING INSTRUCTIONS

Instrument Description

The Xenogen IVIS-200 System is a high-sensitivity, low noise, in vivo imaging technology platform that allows non-invasive visualization and tracking of cellular and genetic activity within a living organism, in real time. It’s capable of imaging bioluminescence and fluorescence. IVIS-200 provides Living Image® software for image display and analysis.

Instrument Specifications

  • CCD Cameras
    • Sensor
      • Back Illuminated
    • CCD Format
      • 2048 x 2048 pixels
    • CCD Size
      • 26 x 26 mm
    • Effective CCD Format
      • 1920 x 1920 pixels
    • Pixel Size
      • 13.5 x 13.5 um
    • Quantum Efficiency
      • ~85% 400-700 nm
        • >50% 350-900 nm
      • Readout Noise
        • <5 electrons RMS
      • Dark Current
        • <100 electrons/s/cm2 (-105°c)
      • Minimum Detectable Luminance
        • <70 photons/s/sr/cm2
      • CCD Temperature
        • Nominal ~105°C
    • Optics
      • Lens f/stop
        • f/1~f/8
      • Field of views (FOV)
        • 3.9, 6.5, 13, 19.5 and 26 cm
      • Resolution
        • >60 um (FOV=3.9, f/1)
      • Scanning Laser
        • <1 mW at 532 nm
    • Fluorescence Imaging Components
      • Excitation Filters
        • 6 positions, 25 mm diameter
      • Emission Filters
        • 22 positions, 60 mm diameter
      • Background (Autofluorescence and Leakage)
        • 1 part in 107 typical
      • Lamp
        • 150 W Quartz Halogen 21 V 3250° Kelvin

    There are fluorescence filter sets available on the system are as follows:

    Filter Set

    Label

    Excitation

    Emission Passband

    Dyes & Passband

    1GFP445-490515-575GFP, EGFP, FITC
    2DsRed500-550575-650DsRed2-1, PKH26, CellTrackerOrange
    3Cy5.5615-665695-770Cy5.5, Alexa Fluor® 660, Alexa Fluor® 680
    4ICG710-760810-875Indocyanine green (ICG)