Leica SP8 STED Super Resolution Confocal

Eckstein Medical Research Building - Room 89

The Leica 3x STED system is a super resolution microscope based on the Leica SP8 confocal microscope. It has a guaranteed resolution of 50 nm though the system has been routinely achieving 40 nm. It is equipped with multiple excitation lasers including a continuum fiber laser that can deliver up to 8 simultaneous lines of any wavelengths between 470 and 670 nm. All common fluorophores can be imaged. Alexa 532 and tetramethylrhodamine are ideal STED fluorophores. YFP, Venus and mStrawberry are excellent STED fluorescent proteins. It has 4 detectors, two of which are high-sensitivity Hybrid detectors. It can be operated in STED or standard confocal mode. Feature highlights include:

  •  Continuum Fiber Laser: continuous excitation  tuning from 470 nm to 670 nm
  • Argon Ion Laser with 457, 477, 488 and 514 nm lines
  • 405 nm DPSS laser
  • 660 nm Depletion laser
  • Two HyD and two PMT detectors
  • Transmitted light detector
  • Objective lenses
    • 10x dry
    • 20x dry
    • 40x oil for confocal
    • 63x oil for confocal
    • 100x oil for STED only


Useful Links


Basic Instructions

STED theory and labeling strategies 

STED Sample Prep Quick Guide

Acceptor Photobleaching FRET Guide

Sensitized Emission FRET Guide

FRAP Guide

Link to Carver College of Medicine STED article


STED Inventor Stefan Hell wins Nobel Prize!