Instrumentation

The Central Microscopy Research Facilities offer investigators a variety of microscopes and preparation equipment to support a wide range of imaging techniques.

Acknowledgement of the CMRF and of NIH Shared Instrumentation Grants in Publications

Reviews of new applications for NIH-funded shared instrumentation grants (SIG) now include an evaluation of the track record of usage of instruments supported by previous SIG awards. For this purpose, SIG applications now require inclusion of a table listing the previous SIG-funded instruments awarded to The University of Iowa with quantification of the publications that specifically acknowledged the awarded SIG grant. When publishing, we encourage you to acknowledge the CMRF, the instrument and the NIH grant number in the paper. Doing so will improve the chances of the CMRF acquiring additional state-of-the-art equipment in the future. Specifically:

The NIH requires that all grantee publications include the following two statements:

1) A specific acknowledgment of NIH grant support, such as:
"Research reported in this [publication/press release] was supported by [name of the Institute(s), Center, or other NIH offices] of the National Institutes of Health under award number [specific NIH grant number(s) in this format: R01GM987654]." You should also include the name of the instrument.

In the case of publishing data acquired on CMRF equipment you should include a statement such as "This work utilized the “Name of Equipment” in the University of Iowa Central Microscopy Research Facilities that was purchased with funding from the NIH SIG grant “grant number for instrument from the list below.

2) A disclaimer that says:
"The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."

Current CMRF instruments that have been acquired with funding from the National Institutes of Health Shared Instrumentation Grants are listed below:

Grant Number Equipment
1 S10 RR025439-01 Zeiss LSM710 confocal
1 S10 OD014165-01A1 Ariol Slide-Scanning Microscope & MicroBrightfield Stereology Software 
1 S10 OD016316-01   Leica LMD7000 Laser Capture Microdissection System
1 S10 RR022498-01     Hitachi S-4800 & Hitachi S-3400N Scanning Electron Microscopes
1 S10 RR018998-01 JEOL JEM-1230 Transmission Electron Microscope
1 S10 RR12916-01                             Zeiss LSM510 confocal

If you are a member of the Holden Comprehensive Cancer Center, when publishing you should also acknowledge the Cancer Center support grant P30CA086862.

Additionally, in order to support the future procurement of new laboratory equipment, please review our acknowledgement and co-authorship policy.

All equipment Instruction guides are in .pdf format. The following operation guides are NOT a substitution for training. If you need to schedule a time to train with a staff member, please contact the CMRF at (319) 335-8142.

Transmission Electron Microscopy

Scanning Electron Microscopy

Light Microscopy

Confocal/Super Resolution Microscopy

Surface Science

Sample Preparation

  • BAL-TEC High Pressure Freezing 010
  • Edward Vacuum Coater Auto306
  • Emitech Sputter Coater K550
  • Quorum Technologies EMS 150 T ES iridium Sputter Coater
  • Histotronix Tissue-Tek
  • Cryo Console
  • Dispensing Console
  • Thermal Console
  • Leica Automatic Freeze Substitution (AFS)
  • Leica LMD 7000
  • Leica Microm Cryostat I HM505E
  • Leica Microm Cryostat II w/ Cryo-Jane System
  • Leica Microtome RM2135
  • Leica EM UC6 Ultramicrotome
  • Leica EM UC6 Ultramicrotome w/ Cryo Attachment FC6
  • Microm Microtome HM355
  • Pelco BioWave Microwave
  • Polaron Critical Point Dryer (CPD)
  • Reichert-Jung Quick Freeze KF80
  • RMC Paraffin Tissue Processor 1530
  • Shandon Cyto-Spin 3
  • Sledge Microtome
  • Sakura Diversified Stainer DRS-601
  • Spencer Microtome
  • Vibratome Automated 1500

Image Analysis