Leica SP8 STED Super Resolution Microscope

The Leica 3x STED system is a super resolution microscope based on the Leica SP8 confocal microscope. It has a guaranteed resolution of 50 nm though the system has been routinely achieving 40 nm. It is equipped with multiple excitation lasers including a continuum fiber laser that can deliver up to 8 simultaneous lines of any wavelengths between 470 and 670 nm. All common fluorophores can be imaged. Alexa 532 and tetramethylrhodamine are ideal STED fluorophores. YFP, Venus and mStrawberry are excellent STED fluorescent proteins. It has 4 detectors, two of which are high-sensitivity Hybrid detectors. It can be operated in STED or standard confocal mode. Feature highlights include:

  •  Continuum Fiber Laser: continuous excitation  tuning from 470 nm to 670 nm
  • Argon Ion Laser with 457, 477, 488 and 514 nm lines
  • 405 nm DPSS laser
  • 660 nm Depletion laser
  • Two HyD and two PMT detectors
  • Transmitted light detector
  • Objective lenses
    • 10x dry
    • 20x dry
    • 40x oil for confocal
    • 100x oil for STED only

Basic Instructions

 

STED theory and labeling strategies 

 

Link to Carver College of Medicine STED article

 

STED Inventor Stefan Hell wins Nobel Prize!

http://www.the-scientist.com/?articles.view/articleNo/41169/title/Nanoscopy-Wins-Nobel/